Core Manager
Satoru Kondo
Project Associate Professor
Mission:
The Imaging Core enables comprehensive data acquisition on brain structure and function at different spatial and temporal resolutions using our advanced optical imaging equipment.
Technology:
The Imaging Core provides the highest level of microscope equipment in the country. Observations of fixed brains, cultured cells, and even cells in the living mouse brain can be examined with various spatio-temporal resolutions ranging from submicron to whole brains.
It is an inverted type confocal laser microscope system. This microscope has a large FOV size of 25 and is equipped with a resonant scanner and laser unit LU-N4 (405, 488, 561 and 640 nm), and GaAsP detector. They enable to obtain images with high-speed and high-sensitivity. In addition, culture system (made by Tokai Hit) is also provided, and long-time live cell imaging is possible.
https://www.olympus-lifescience.com/en/microscopes/macro/mvx10/
It is an upright type multiphoton laser microscope system. High sensitivity and high quality images can be acquired with the GaAsP NDD unit. By using piezo elements to drive the objective lens in the Z-axis direction, it is possible to take 3D images at high speed. The femtosecond pulse laser can simultaneously use two laser lines with a variable wavelength of 700-1300 nm and a fixed wavelength of 1040 nm, and is suitable for long-wavelength dye and multicolor imaging.
Virtual Reality (VR) systems can be used with the mouse to investigate brain function during behavioral tasks. You can examine the basic mechanisms of animal navigation, recognition, learning, or memory combined with two-photon and wide-field microscopes.
The patch clamp amplifier supports dual recording. In addition, the manipulator can be driven in 3 axis directions + diagonal direction. We are preparing for in vivo patch clamps, so you need to prepare by yourself for performing in vitro experiments such as slice patches.
It is a fluorescence microscope that does not require a dark room. With the sectioning function, you can obtain clear images without fluorescent blur with easy operation. In addition, high resolution wide field of view image can be taken at high speed by image joint function.
https://www.leica-microsystems.com/products/confocal-microscopes/p/leica-tcs-sp8-sted-one/
Rapid-Scope is a custom-designed light-sheet fluorescence microscope (LSFM) with mesoscopic magnitude (0.5X-2X) to explore a volumetric sample by less-invasive optical slicing. The cleared brain sample is dipped in the oil and illuminated by spatially-uniform thin light-sheet which provides a three-dimensionally isotropic resolution and covers a wide area of visualization.
There are currently no scheduled events.
Past events are here
Usage Application Form
Carry-in laboratory equipment application form
Terms of Use
Rules of the microscope room usage
Important Notice Regarding the Observation of Fixed Samples
Rules for bringing animals to Imaging Core
Microbiological monitoring items
Pledge -Ultra-sound-Imaging-system
Pledge -Ultra-Wide-Two-photon-microscope